OPTIMIZATION OF PHYSICAL AND NUTRITIONAL FACTORS FOR ENHANCED
PRODUCTION OF LIGNIN PEROXIDASE BY GANODERMA LUCIDUM IBL-05 IN
SOLID STATE CULTURE OF WHEAT STRAW
S. Batool, M. Asgher, M. A. Sheikh and S. U. Rahman
Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad.
Corresponding Author E. mail: mabajwapk@yahoo.com
ABSTRACT
Microorganisms are commonly used in biotechnological and environmental processes through exploitation of their
natural catalytic activities. An indigenous white rot fungus Ganoderma lucidum IBL-05 was used in solid state
fermentation
C. By
optimization different physical and nutritional factors, LiP production by the fungus was substantially improved to 1019
IU/mL after 48h in nutrient Medium III with 60% moisture level, 5mL inoculum size, glucose and urea as carbon and
nitrogen sources in 15:1 C/N ratio, 1mL of 2mM Zn
2+
as metal ion and 1mL of 4mM 4-MMA as mediator. Surfactants
like Tween-80, Tween-20 and SDS suppressed LiP synthesis by G. luucidum IBL-05.
Keywords: Ganoderma lucidum IBL-05, Wheat straw, Lignin peroxidase |