ISOLATION AND IDENTIFICATION OF CELLULOSE-DECOMPOSER FUNGUS PENICILLIUM OXALICUM AND DEHYDRATION EFFECTS OF ANALOG COMPOSTS
F. X. Zhua, Y. L. Yaoa, H. Z. Weib, C. L. Honga, L. F. Pinga, Z. Y. Xuea, X. Y. Chena, Y. K. Yanga and W. P. Wanga*
aZhejiang Academy of Agricultural Sciences, Hangzhou 310021,China,
bNinghai County, Agricultural Technique Extension Center, Ninghai, Zhejiang 315600,China
*Corresponding author: E-mail: wangweiping119@126.com
ABSTRACT
The aim of this study was to screen cellulose-decomposer microorganisms and to study the effects of dehydration in simulated composting. Cellulose-decomposer microorganisms were first isolated from fresh cow dung and cow dung natural compost in different stages by the cellulose-Congo Red medium. The obtained strains were further screened for a high ability to decompose cellulose by detecting the cellulose-enzyme activity and by observing the disintegration of filter paper and straw. A strong cellulose-decomposer microorganism was finally obtained and was identified as Penicilliumoxalicum F12. The following aspects were experimentally determined to be the optimal conditions: a wheat bran and microcrystalline cellulose mix (4:1) as the carbon source, ammonium sulfate as the nitrogen source, pH of 5~6, 5% inoculum size, incubation at 30~35ºC for 120h. Under these conditions, the CMCase of strain F12 was 47.50 IU·mL-1, and the FPA was 11.10 IU·mL-1. In a dehydration test of simulated composting, the water content of the inoculated processing residue material was decreased by 3.9% as compared with that of the control residue materials. The dehydration volume was 47.6g, which accounted for 19.8% of the initial material moisture content. Penicilliumoxalicum F12 was a strong cellulose-decomposer microorganism with broad development potential.
Keywords: cellulose-decomposer microorganism; Penicilliumoxalicum; simulated composting
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