CHANGES IN THE EXPRESSION OF ADIPONECTIN AND ITS RECEPTORS (ADIPOR1 AND ADIPOR2) IN SEXUALLY IMMATURE AND SEXUALLY MATURE SHEEP TESTES
Man Bai1, Limin Sun, Jia Zhao, Guishan Zhang, Wei Ma, Lujie Xiang, Jiarong Li, Chao Jia, and Huaizhi Jiang*.
Collage of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
*Corresponding Author: Huaizhi Jiang, E-mail: sheepandgoatgbr@126.com
ABSTRACT
Recent evidences have indicated that adiponectin and its receptors have an effect on male reproduction. The objective of this study was to detect the expression of adiponectin, AdipoR1 and AdipoR2 in sexually immature and sexually mature sheep testes using qRT-PCR and Western blot. The results showed that adiponectin, AdipoR1 and AdipoR2 were all expressed in sheep testes. The expression of AdipoR1and AdipoR2 mRNAs (p<0.05 and p<0.01) as well as the expression of AdipoR1 and AdipoR2 proteins (p<0.01 and p<0.05) in sexually mature testes were significantly higher than that in sexually immature testes. However, there was no significant difference in the expression of adiponectin mRNA (p>0.05) in sexually immature and sexually mature testes, the expression of adiponectin protein in sexually mature testes was significantly higher than that in sexually immature testes (p<0.01). It is the first time to compare the mRNA and protein expression of adiponectin and its receptors in sexually immature and sexually mature sheep testes.
Key words: adiponectin, AdipoR1, AdipoR2, sheep, testes, qRT-PCR, Western-blot |