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ANTI-OXIDATIVE EFFECT OF L-LYSINE ON POST THAW QUALITY OF NILI-RAVI BUFFALO BULL SEMEN (BUBALUS BUBALIS)
A. Ahmed1, M. Ayaz1, D. H. Mughal2, A. Rehman3, M. H. Mushtaq3, M. S. Yousaf4, B. U. Din5 and A. Riaz1,*
1Department of Theriogenology, University of Veterinary and Animal Sciences, Lahore, Pakistan
2Directorate of Quality Enhancement Cell, University of Veterinary and Animal Sciences, Lahore, Pakistan
3Department of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore, Pakistan
4Department of Physiology, University of Veterinary and Animal Sciences, Lahore, Pakistan
5Cattle Breeding and Dairy Farm, Harichand, Charsadda, KPK, Pakistan
*Corresponding author’s E-mail: dramjadriaz@uvas.edu.pk
The paper was presented in International Buffalo Congress 2019, February 18-20, Lahore, Pakistan
ABSTRACT
This study was designed to investigate the effect of L-lysine on post-thaw quality of Nili-Ravi buffalo bull semen. Twenty eight ejaculates were collected from four Nili-Ravi buffalo bulls. Semen samples were pooled and split into five equal aliquots and diluted in Tris-citric acid extender containing different concentrations of L-lysine (0.25, 0.5, 1 and 2mM). As a control, semen was extended without addition of L-lysine. The semen samples were cryo-preserved in liquid nitrogen. Post-thaw semen assessment revealed that sperm motility, viability, plasma membrane integrity, acrosomal integrity, mitochondrial integrity and DNA integrity were significantly improved (P ≤ 0.05) in 2mM L-lysine supplemented group as compared to control. For other groups supplemented with L-lysine; post-thaw motility, acrosomal integrity and mitochondrial integrity were also improved (P ≤ 0.05) for 1mM treated group as compared to control. DNA integrity was improved significantly by all L-lysine treated groups as compared to control. Malondialdehyde concentration was higher (P ≤ 0.05) in control group as compared to L-lysine treated groups. Significantly higher rate of catalase was recorded for 1mM and 2mM L-lysine treated groups as compared to control. In conclusion, the supplementation of 2mM L-lysine in semen extender can be used for cryopreservation of Nili-Ravi buffalo bull semen.
Keywords: Nili-Ravi, Semen, Cryopreservation, L-lysine, Antioxidant
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