Short communication
EFFECTS OF FASN AND SCD GENE POLYMORPHISM ON THE COMPOSITION OF SHEEP’S MILK
Ewa Czerniawska-Piątkowska1, Inga Kowalewska-Łuczak2,Ewa Pecka-Kiełb3 and Dorota Banaszewska4
1West Pomeranian University of Technology, Laboratory of Molecular Cytogenetics, Klemensa Janickiego 29, 71-270 Szczecin, Poland
2West Pomeranian University of Technology, Department of Genetics and Animal Breeding, Piastów Avenue 45, 70-311 Szczecin, Poland
3Wroclaw University of Environmental and Life Sciences, Department of Biostructure and Animal Physiology, Norwida 31, 50-375 Wroclaw, Poland
4Siedlce University of Natural Sciences and Humanities, Faculty of Agrobioengineering and Animal Husbandry, Institute of Animal Science and Fisheries, Prusa 14 St, 08-110 Siedlce, Poland
*Corresponding author: ewa.pecka@upwr.edu.pl
ABSTRACT
In the study, genotypes and allele frequencies for SNP of FASN and SCD1 genes in the herd of Zošľachtená Valaška sheep in the second and third lactation were determined. In the obtained samples, the basic composition of milk, urea content and proportion of protein fractions were estimated. Blood samples were tested to determine FASN and SCD1 genes frequencies. After genotyping conducted in the sheep herd, three possible genotypes in SCD1/CfrI polymorphism and only two out of three possible genotypes in SCD1/PvuII and FASN/AciI polymorphisms (AA, AG and CC, CT, respectively) were found. Analyses have shown changes in milk composition and proportions of protein fractions in FASN and SCD1 genes depending on SNP genotypes. The analysis of the obtained results for FASN polymorphism allowed to establish that the milk of animals with CT genotype had the highest content of protein, fat and dry matter. The most desired profile of protein fractions was found in SCD/CfrI genotype among the homozygous CC genotype carriers: it allows to determine that the milk of these sheep has good technological traits.
Key words: sheep, milk composition, FASN , SCD1 |