Removal of early fruiting branches with optimum nitrogen dose caused more source and less sink at early stages leading to delay in onset and progression of senescence in cotton. Field trials were conducted to investigate the effects of squares and fruiting branches removal under different nitrogen levels on the growth of Bt cotton at Students’ Farm, University of Agriculture Faisalabad, during 2011 and 2012. Experiment was laid out in randomized complete block design (RCBD) with factorial arrangement using three replications. Treatments comprised of manual alteration of plant architecture (F1: no fruiting branch removal, F2: removal of first fruiting branch, F3: removal of first and second fruiting branch, F4: removal of all squares from first fruiting branch, F5: removal of all squares from first and second fruiting branch) and nitrogen rates (N1: 175, N2: 225 and N3: 275 kg ha-1). Results exhibited more number of nodes above white flower (NAWF) recorded in F5, followed by F3, F4 and F2 while minimum NAWF recorded in F1. Among nitrogen levels maximum nodes above white flower were recorded in N3 followed by N2 and N1 during both years of study. Before manual alteration of the plants architecture, no variation in plant height was observed at squaring stage, but at physiologically cut-out stage plants gained more height with removal of squares/fruiting branches with maximum level of nitrogen. Shorter boll maturation period was recorded in F4 and F5 than in F1, F2 and F3. Lower earliness index was observed in F5 and F3 and higher earliness index in F1. Longer boll maturation period, earliness index and seed index were recorded with 275 kg N ha-1. Removal of first and second fruiting branch and removal of all squares from first and second fruiting branch along with higher nitrogen dose helped in delayed onset of senescence in cotton leading to improved translocation of assimilates towards economic part and thus more seed cotton yield (data not given).