17 E. EL-Mohsnawy*1 M. M. El-Sheekh2 M. E.M. Mabrouk3 and W. F. Zohir3 Journal of Animal and Plant Sciences JAPS 20202020/03/02 30 2 485-492 1018-7081 https://doi.org/10.36899/JAPS.2020.2.0047 <p>Omega fatty acids have critical part in enhancing general health and protection against serious diseases. The present work showed the distinctive nutritional regimes&nbsp;on&nbsp;<em>Chlorella vulgaris</em>&nbsp;which resulted in switch of metabolic pathways that not only prompted the dry weight and lipid yield, but also upgraded the yield&nbsp;of special fatty acids, omega 3 and 9 fatty acids, which consequently improved the food quality for human and aqua-creatures. High biomass and lipid production were accomplished by either cultivating&nbsp;<em>C. vulgaris</em> at low nitrogen content medium and/or mixotrophic nutrition by glycerol or sugarcane molasses. Glycerol supplementation promoted the dry weight achieving 171% at 0.1 M and 185% at 0.2 M, while the highest total lipid yield (179%) recorded at 0.1 M. GC&ndash;MS qualitative and quantitative analysis showed the high yield of &alpha;-Linolenic acid&nbsp;to 281%, Eicosapentaenoic acid to 207% at glycerol (0.1 M). Elaidic acid (C18:1t &omega;9) enhanced almost 14 times, while Erucic acid (C22:1 &omega;9) enhanced to 256% under glycerol supplementation to growth medium.</p> Chlorella vulgaris, Omega 3, 9, mixotroph, fatty acids, gas chromatography Pakistan Agricultural Scientists Forum https://thejaps.org.pk/AbstractView.aspx?mid=Bioch-19-0004