RT Journal
T1 BIODEGRADATION OF SYNTHETIC TEXTILE DYES BY CHITOSAN BEADS CROSS-LINKED LACCASE FROM Pleurotus ostreatus IBL-02
A1 F. Jamil
A1 M. Asgher
A1 F. Hussain and H. N. Bhatti
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 28
IS 1
SP 231
OP 243
YR 2018
FD 2018/02/01
DO DOI NA
AB <p>Laccase (EC.1.10.3.2) a blue copper protein is remarkable, ecologically well disposed and productive biocatalyst that can degrade the toxic contaminants. The utilization of laccase for the detoxification of toxic pollutants is extensively restricted by its vulnerability to changes in operational conditions and its poor reusability. To overcome this problem, indigenously produced laccase from&nbsp;<em>Pleurotus ostreatus</em>&nbsp;IBL-02 was immobilized using glutaraldehyde as cross-linker. Maximum immobilization efficiency (76.2%) was noticed with 3% Chitosan, cross-linked by 3% solution of glutaraldehyde. Cross-linking significantly altered the kinetic parameters&nbsp;<em>K</em>m and&nbsp;<em>V</em>max of laccase. The affinity of substrate towards biocatalyst improved (<em>K</em>m decreased) consequently upgraded catalytic activity (increased&nbsp;<em>V</em>max). Immobilization significantly raised the temperature optima while shifted the optimum pH towards slightly neutral from acidic. The presence of laccase in immobilized beads was also confirmed by Energy Dispersive X-ray (EDX) analysis. Immobilized laccase was used to decolorize five different synthetic textile dyes. Chitosan cross-linked laccase decolorized all the dyes more efficiently as compared to control.&nbsp;&nbsp;</p>
K1 Laccase, Chittosan, Cross-linking, Immobilization, Biodegradation
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2018-JAPS-29